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The organizational meeting of the Light Microscopy Special Interest Group was held on Jan. 13, 2000. The group decided the following:
Interest Group Format:
1. Monthly topic based meetings:
The meetings will be organized by a group member interested in the particular topic, who will arrange for 2-3 mainly local "experts" to talk about the technique and how they applied it to their research. The presentations should be informal, short. Speakers are encouraged to talk about how the technique worked in their hands including failures. The organizer should give a short overview of the technique or field which is the topic of the meeting. At the end,meeting members are welcome to stay for an open discussion on problems unrelated to the topic of the meeting.
2. Yearly social gathering for interest group members, potentially associated with an invited outside speaker.
3. Small vendor fairs on special topic (perhaps twice per year)
4. The group will maintain a website with technical support from CIT including a Resource directory, listing microscope facilities and group members with their area of expertise.
5. The Interest group list server shall not solely be used for meeting announcements but shall also be open for discussions of microscopy problems/questions, equipment advice . A FAQ based on the listserv discussion shall be maintained on the Interest group website with an appropriate disclaimer that these represent opinions of individuals and not an official government position.
6. A subgroup of the interest group will prepare and run a basic microscopy tutorial open to NIH research personnel interested in microscopy. The main goal of the tutorial is to educate core facility users and microscope novices in basic light microscopy techniques and digital imaging.
Time and location of monthly meetings:
The LMIG meets monthly on a Tuesday at 12 noon in Bldg 10, Rm 4B51.
Topics of interest for future meetings of the interest group:
- TIRF (total internal reflection microscopy)
- FLIM (fluorescence lifetime imaging microscopy)
- FRAP (fluorescence recovery after photobleaching)
- PAF (photo-activated fluorescence)
- FRET (fluorescence resonance energy transfer)
- FCS (fluorescence correlation spectroscopy)
- GFP / dual GFP
- 2photon
- LM and EM (integration of light and electron microscopy)
- laser tweezers
- new dyes
- deconvolution
- grid based confocal imaging / extended depth of field microscopy
- near field microscopy
- image analysis
- morphometric analysis
- spectroscopy/spectral scanning
- illumination systems
- image databases
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| General Microscopy Resources |
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A Cellular Observatory at Pacific Northwest National Lab |
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Includes a description of a number of custom microscopes that combine an assortment of technologies, including patch clamp and light microscopy, Raman scattering and two photon microscopy and magnetic resonance and light microscopy. The center seeks collaboration with biologists who will benefit from this microscopy instrumentation, or who have ideas for other microscope developments. |
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Confocal Listserver |
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Email discussion list focused on confocal microscopy, but also including topics on fluorescence microscopy and digital imaging. |
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Microscopy Listserver |
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Email discussion list on LM, SEM, and TEM, maintained by the Microscopy Society of America. |
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Molecular Expressions: Exploring the World of Optics and Microscopy |
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Comprehensive descriptions and tutorials on many types of microscopy |
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| NIH Microscopy Resources |
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ImageJ |
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Java-based, platform independent image processing tool developed by Wayne Rasband |
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NCI Microscopy Core Facilities |
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Contact and equipment information of NCI microscopy core facilities |
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NHLBI Light Microscopy Imaging Facility |
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Web Page includes descriptions of available facility equipment, instructions for using the facility, and general core information |
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NIH Image |
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Image processing software for MacOS developed by Wayne Rasband at NIH |
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